Ovicidal Effect on Haemonchus contortus of Extract Partitions Shrubby Plants of the Tropical Dry Forest and Potentially Active Compounds Identification by UHPLC-Q/Orbitrap/MS/MS

The in vitro anthelmintic effect of the extracts on Haemonchus contortus (H. contortus) three forage species tropical dry forest is known; however, there no information about effects extract partitions, nor their chemical composition. objectives this study were to evaluate ovicidal activity H. partitions Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce, identify compounds present with highest by employing ultra HPLC Quadrupole orbitrap mass spectrometry. Four hexane, dichloromethane, ethyl acetate, hydroethanolic from assessed an inhibition egg hatching (IEH) assay. (AA) subjected analysis, which tentative identification was established. including dichloromethane acetate dulce showed a greater effect, IC50 values 0.39, 0.86, 0.27 mg/mL for IEH, respectively. Metabolites AA potential included flavonoids, fatty acid esters, hydroxycinnamic acids, organic oxygenated benzene class substituted derivatives, phenolic glycosides, phenols.